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Assay Detail

CHEMBL5147544

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV-2 main protease expressed in Escherichia coli using DABCYL-KTSAVLQ1SGFRKM-E(EDANS)-NH2 peptide as substrate incubated for 30 mins by FRET based assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Identification of Aloe-derived natural products as prospective lead scaffolds for SARS-CoV-2 main protease (M<sup>pro</sup>) inhibitors.
Hicks EG, Kandel SE, Lampe JN.
Bioorg Med Chem Lett (2022) 66 : 128732 -128732
PubMed 35427739 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.79 5.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL33778 ANACARDIC ACID 1 5.17
CHEMBL5172191 1 4.41
CHEMBL1241028 1 -
CHEMBL5187969 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL33778 ANACARDIC ACID IC50 = 6800.0 nM 5.17
CHEMBL5172191 IC50 = 38900.0 nM 4.41
CHEMBL1241028 IC50 = 138000.0 nM -
CHEMBL5187969 IC50 = 125300.0 nM -