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Assay Detail

CHEMBL5154109

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Mycobacterium smegmatis MC2 155 recombinant ndhA assessed as rate of NADH oxidation in the presence of an electron acceptor menadione
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mycolicibacterium smegmatis
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Type II NADH:quinone oxidoreductase NdhA (CHEMBL5169229)
Type SINGLE PROTEIN
Organism Mycobacterium tuberculosis

Publication

Tricyclic SpiroLactams Kill Mycobacteria In Vitro and In Vivo by Inhibiting Type II NADH Dehydrogenases.
Dam S, Tangara S, Hamela C, Hattabi T, Faïon L, Carre P, Antoine R, Herledan A, Leroux F, Piveteau C, Eveque M, Flipo M, Deprez B, Kremer L, Willand N, Villemagne B, Hartkoorn RC.
J Med Chem (2022) 65 : 16651 -16664
PubMed 36473699 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.60 7.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5186518 1 7.24
CHEMBL5201484 1 7.23
CHEMBL5171834 1 7.02
CHEMBL5202255 1 6.48
CHEMBL5182198 1 6.01
CHEMBL5206556 1 5.63
CHEMBL5196810 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5186518 IC50 = 57.0 nM 7.24
CHEMBL5201484 IC50 = 59.0 nM 7.23
CHEMBL5171834 IC50 = 96.0 nM 7.02
CHEMBL5202255 IC50 = 330.0 nM 6.48
CHEMBL5182198 IC50 = 980.0 nM 6.01
CHEMBL5206556 IC50 = 2340.0 nM 5.63
CHEMBL5196810 IC50 - -