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Assay Detail

CHEMBL5159130

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human GST-Xa-tagged-AAK1 (30 to 330 residues) using (5-FAM)-Aha-KEEQSQITSQVTGQIGWR-NH2 as substrate incubated for 3 hrs in presence of ATP by electrophoretic analysis
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

AP2-associated protein kinase 1 (CHEMBL3830)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Bicyclic Heterocyclic Replacement of an Aryl Amide Leading to Potent and Kinase-Selective Adaptor Protein 2-Associated Kinase 1 Inhibitors.
Hartz RA, Ahuja VT, Nara SJ, Kumar CMV, Manepalli RKVLP, Sarvasiddhi SK, Honkhambe S, Patankar V, Dasgupta B, Rajamani R, Muckelbauer JK, Camac DM, Ghosh K, Pokross M, Kiefer SE, Brown JM, Hunihan L, Gulianello M, Lewis M, Lippy JS, Surti N, Hamman BD, Allen J, Kostich WA, Bronson JJ, Macor JE, Dzierba CD.
J Med Chem (2022) 65 : 4121 -4155
PubMed 35171586 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.11 8.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5194899 1 8.38
CHEMBL5202351 1 8.24
CHEMBL4543869 1 8.21
CHEMBL5195909 1 8.14
CHEMBL5182431 1 7.96
CHEMBL4872886 1 7.92
CHEMBL5201888 1 7.92
CHEMBL5205801 1 7.80
CHEMBL5175828 1 7.77
CHEMBL5207636 1 7.68
CHEMBL5204563 1 7.66
CHEMBL5170931 1 7.62
CHEMBL5208293 1 7.52
CHEMBL5200458 1 7.36
CHEMBL5182634 1 7.17
CHEMBL5204415 1 7.02
CHEMBL5174061 1 6.96
CHEMBL4862414 1 6.77
CHEMBL5184309 1 6.77
CHEMBL5209199 1 6.68
CHEMBL5177481 1 6.66
CHEMBL5193040 1 6.64
CHEMBL5176849 1 6.47
CHEMBL5171538 1 6.44
CHEMBL5208680 1 6.44
CHEMBL5187603 1 6.40
CHEMBL5191369 1 6.38
CHEMBL5172186 1 6.38
CHEMBL5197898 1 5.96
CHEMBL5179127 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5194899 IC50 = 4.2 nM 8.38
CHEMBL5202351 IC50 = 5.8 nM 8.24
CHEMBL4543869 IC50 = 6.2 nM 8.21
CHEMBL5195909 IC50 = 7.3 nM 8.14
CHEMBL5182431 IC50 = 11.0 nM 7.96
CHEMBL4872886 IC50 = 12.0 nM 7.92
CHEMBL5201888 IC50 = 12.0 nM 7.92
CHEMBL5205801 IC50 = 16.0 nM 7.80
CHEMBL5175828 IC50 = 17.0 nM 7.77
CHEMBL5207636 IC50 = 21.0 nM 7.68
CHEMBL5204563 IC50 = 22.0 nM 7.66
CHEMBL5170931 IC50 = 24.0 nM 7.62
CHEMBL5208293 IC50 = 30.0 nM 7.52
CHEMBL5200458 IC50 = 44.0 nM 7.36
CHEMBL5182634 IC50 = 67.0 nM 7.17
CHEMBL5204415 IC50 = 95.0 nM 7.02
CHEMBL5174061 IC50 = 110.0 nM 6.96
CHEMBL4862414 IC50 = 170.0 nM 6.77
CHEMBL5184309 IC50 = 170.0 nM 6.77
CHEMBL5209199 IC50 = 210.0 nM 6.68
CHEMBL5177481 IC50 = 220.0 nM 6.66
CHEMBL5193040 IC50 = 230.0 nM 6.64
CHEMBL5176849 IC50 = 340.0 nM 6.47
CHEMBL5171538 IC50 = 360.0 nM 6.44
CHEMBL5208680 IC50 = 360.0 nM 6.44
CHEMBL5187603 IC50 = 400.0 nM 6.40
CHEMBL5191369 IC50 = 420.0 nM 6.38
CHEMBL5172186 IC50 = 420.0 nM 6.38
CHEMBL5197898 IC50 = 1100.0 nM 5.96
CHEMBL5179127 IC50 = 1800.0 nM 5.75
CHEMBL5193199 IC50 = 5300.0 nM 5.28