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Assay Detail

CHEMBL5162303

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type human N-terminal GST tagged EGFR (669 to 1210 residues) expressed in Sf21 insect cells incubated for 30 mins in presence of ATP by HTRF analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of SPH5030, a Selective, Potent, and Irreversible Tyrosine Kinase Inhibitor for HER2-Amplified and HER2-Mutant Cancer Treatment.
Li D, Tu Y, Jin K, Duan L, Hong Y, Xu J, Chen N, Zhang Z, Zuo H, Gong W, Zhang J, Wang Q, Qian H, Wang X, Ke Y, Xia G.
J Med Chem (2022) 65 : 5334 -5354
PubMed 35319895 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 8.18 9.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL180022 NERATINIB 4.0 1 9.24
CHEMBL3647420 PYROTINIB 3.0 1 9.01
CHEMBL5190132 1 8.99
CHEMBL5176920 1 8.96
CHEMBL5179954 1 8.92
CHEMBL5184610 1 8.87
CHEMBL5175489 1 8.83
CHEMBL5175873 1 8.67
CHEMBL5192750 1 8.64
CHEMBL5201973 1 8.63
CHEMBL5193451 1 8.55
CHEMBL5209046 1 8.50
CHEMBL5178703 1 8.09
CHEMBL5183924 1 8.02
CHEMBL5202846 1 7.93
CHEMBL5180725 1 7.68
CHEMBL5180965 1 7.64
CHEMBL5181694 1 7.59
CHEMBL5176854 1 7.49
CHEMBL5170965 1 7.06
CHEMBL5203179 1 6.96
CHEMBL5200783 1 5.74

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL180022 NERATINIB IC50 = 0.58 nM 9.24
CHEMBL3647420 PYROTINIB IC50 = 0.98 nM 9.01
CHEMBL5190132 IC50 = 1.03 nM 8.99
CHEMBL5176920 IC50 = 1.11 nM 8.96
CHEMBL5179954 IC50 = 1.19 nM 8.92
CHEMBL5184610 IC50 = 1.36 nM 8.87
CHEMBL5175489 IC50 = 1.47 nM 8.83
CHEMBL5175873 IC50 = 2.13 nM 8.67
CHEMBL5192750 IC50 = 2.31 nM 8.64
CHEMBL5201973 IC50 = 2.32 nM 8.63
CHEMBL5193451 IC50 = 2.8 nM 8.55
CHEMBL5209046 IC50 = 3.13 nM 8.50
CHEMBL5178703 IC50 = 8.13 nM 8.09
CHEMBL5183924 IC50 = 9.6 nM 8.02
CHEMBL5202846 IC50 = 11.82 nM 7.93
CHEMBL5180725 IC50 = 20.72 nM 7.68
CHEMBL5180965 IC50 = 22.75 nM 7.64
CHEMBL5181694 IC50 = 25.92 nM 7.59
CHEMBL5176854 IC50 = 32.14 nM 7.49
CHEMBL5170965 IC50 = 86.33 nM 7.06
CHEMBL5203179 IC50 = 110.32 nM 6.96
CHEMBL5200783 IC50 = 1833.8 nM 5.74