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Assay Detail

CHEMBL5163411

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at ERbeta expressed in HEK293/Gal4 cells incubated for 24 hrs in presence of di-arylpropionitrile by luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor beta (CHEMBL242)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Thieno[2,3-<i>e</i>]indazole Derivatives as Novel Oral Selective Estrogen Receptor Degraders with Highly Improved Antitumor Effect and Favorable Druggability.
Lu Z, Cao Y, Zhang D, Meng X, Guo B, Kong D, Yang Y.
J Med Chem (2022) 65 : 5724 -5750
PubMed 35357160 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 8.73 9.52
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1358 FULVESTRANT 4.0 1 9.52
CHEMBL5197035 1 8.70
CHEMBL5185539 1 8.64
CHEMBL4475463 AMCENESTRANT 3.0 1 8.07
CHEMBL5181850 1 -
CHEMBL5171948 1 -
CHEMBL5205546 1 -
CHEMBL5191161 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1358 FULVESTRANT IC50 = 0.3 nM 9.52
CHEMBL5197035 IC50 = 2.0 nM 8.70
CHEMBL5185539 IC50 = 2.3 nM 8.64
CHEMBL4475463 AMCENESTRANT IC50 = 8.6 nM 8.07
CHEMBL5181850 IC50 - -
CHEMBL5171948 IC50 - -
CHEMBL5205546 IC50 - -
CHEMBL5191161 Activity - -