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Assay Detail

CHEMBL5165218

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Negative allosteric modulation activity against human ATX using FS-3 as substrate preincubated for 45 mins followed by substrate addition and measured every 30 mins by multi-mode microplate reader
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Autotaxin (CHEMBL3691)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and promising anti-tumor efficacy of novel imidazo[1,2-a]pyridine derivatives as potent autotaxin allosteric inhibitors.
Lei H, Wang X, Zhao G, Li T, Cui Y, Wu H, Yang J, Jiang N, Zhai X.
Eur J Med Chem (2022) 236 : 114307 -114307
PubMed 35436669 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.30 8.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5177541 1 8.47
CHEMBL3186509 1 8.43
CHEMBL3828074 ZIRITAXESTAT 3.0 1 8.41
CHEMBL5195787 1 8.15
CHEMBL5189914 1 7.77
CHEMBL5171446 1 7.62
CHEMBL5189398 1 7.58
CHEMBL5174427 1 7.47
CHEMBL5171136 1 7.47
CHEMBL5204000 1 7.39
CHEMBL5194357 1 7.39
CHEMBL5170680 1 7.29
CHEMBL5192468 1 7.15
CHEMBL5179219 1 7.09
CHEMBL5190828 1 7.04
CHEMBL5201210 1 6.89
CHEMBL5200077 1 6.85
CHEMBL5204526 1 6.81
CHEMBL5189263 1 6.79
CHEMBL5175013 1 6.77
CHEMBL5179154 1 6.66
CHEMBL5204769 1 6.63
CHEMBL5200527 1 6.54
CHEMBL5194595 1 6.50
CHEMBL5208395 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5177541 IC50 = 3.4 nM 8.47
CHEMBL3186509 IC50 = 3.7 nM 8.43
CHEMBL3828074 ZIRITAXESTAT IC50 = 3.9 nM 8.41
CHEMBL5195787 IC50 = 7.1 nM 8.15
CHEMBL5189914 IC50 = 17.0 nM 7.77
CHEMBL5171446 IC50 = 24.0 nM 7.62
CHEMBL5189398 IC50 = 26.4 nM 7.58
CHEMBL5174427 IC50 = 34.0 nM 7.47
CHEMBL5171136 IC50 = 33.5 nM 7.47
CHEMBL5204000 IC50 = 41.0 nM 7.39
CHEMBL5194357 IC50 = 41.0 nM 7.39
CHEMBL5170680 IC50 = 51.3 nM 7.29
CHEMBL5192468 IC50 = 71.0 nM 7.15
CHEMBL5179219 IC50 = 81.0 nM 7.09
CHEMBL5190828 IC50 = 91.0 nM 7.04
CHEMBL5201210 IC50 = 129.0 nM 6.89
CHEMBL5200077 IC50 = 140.0 nM 6.85
CHEMBL5204526 IC50 = 154.0 nM 6.81
CHEMBL5189263 IC50 = 164.0 nM 6.79
CHEMBL5175013 IC50 = 171.0 nM 6.77
CHEMBL5179154 IC50 = 217.0 nM 6.66
CHEMBL5204769 IC50 = 234.0 nM 6.63
CHEMBL5200527 IC50 = 287.0 nM 6.54
CHEMBL5194595 IC50 = 313.0 nM 6.50
CHEMBL5208395 IC50 > 1000.0 nM -