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Assay Detail

CHEMBL5165908

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant rat DYRK1A (1 to 499 residues ) expressed in bacterial expression system using KKISGRLSPIMTEQ as substrate measured after 30 mins in presence of ATP by ADP-Glo assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity tyrosine-phosphorylation-regulated kinase 1A (CHEMBL5508)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and biological evaluation of Haspin inhibitors: Kinase inhibitory potency and cellular activity.
Zeinyeh W, Esvan YJ, Josselin B, Defois M, Baratte B, Knapp S, Chaikuad A, Anizon F, Giraud F, Ruchaud S, Moreau P.
Eur J Med Chem (2022) 236 : 114369 -114369
PubMed 35447555 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.73 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5178368 1 7.89
CHEMBL5188318 1 7.33
CHEMBL5186093 1 7.06
CHEMBL5186471 1 6.88
CHEMBL5194011 1 6.83
CHEMBL5196946 1 6.29
CHEMBL5177344 1 6.23
CHEMBL5183366 1 6.04
CHEMBL5181620 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5178368 IC50 = 13.0 nM 7.89
CHEMBL5188318 IC50 = 47.0 nM 7.33
CHEMBL5186093 IC50 = 87.0 nM 7.06
CHEMBL5186471 IC50 = 132.0 nM 6.88
CHEMBL5194011 IC50 = 148.0 nM 6.83
CHEMBL5196946 IC50 = 515.0 nM 6.29
CHEMBL5177344 IC50 = 594.0 nM 6.23
CHEMBL5183366 IC50 = 916.3 nM 6.04
CHEMBL5181620 IC50 = 917.0 nM 6.04