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Assay Detail

CHEMBL5166787

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at wild type human TR alpha LBD expressed in Escherichia coli BL21 (DE3) assessed as induction of N-terminal biotinylated coactivator SRC2-3 peptide recruitment by alphascreen assay
28
Total Activities
28
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Thyroid hormone receptor alpha (CHEMBL1860)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Highly Selective and H435R-Sensitive Thyroid Hormone Receptor β Agonist.
Li Q, Yao B, Zhao S, Lu Z, Zhang Y, Xiang Q, Wu X, Yu H, Zhang C, Li J, Zhuang X, Wu D, Li Y, Xu Y.
J Med Chem (2022) 65 : 7193 -7211
PubMed 35507418 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 7.36 8.32
Activity 7 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5172845 1 8.32
CHEMBL5172205 1 8.05
CHEMBL5209093 1 7.83
CHEMBL5180999 1 7.80
CHEMBL5188839 1 7.75
CHEMBL5207186 1 7.62
CHEMBL5186156 1 7.58
CHEMBL4163070 1 7.53
CHEMBL1544 LIOTHYRONINE 4.0 1 7.53
CHEMBL2338329 ROXADUSTAT 4.0 1 7.49
CHEMBL5179829 1 7.48
CHEMBL5191021 1 7.44
CHEMBL5171808 1 7.41
CHEMBL5207720 1 7.35
CHEMBL5193673 1 7.28
CHEMBL5180738 1 7.25
CHEMBL5207967 1 7.16
CHEMBL5191155 1 7.04
CHEMBL5206520 1 6.57
CHEMBL5171818 1 6.52
CHEMBL3261331 RESMETIROM 4.0 1 5.56
CHEMBL5200322 1 -
CHEMBL5178993 1 -
CHEMBL5178553 1 -
CHEMBL5181604 1 -
CHEMBL5204264 1 -
CHEMBL5205719 1 -
CHEMBL5173831 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5172845 EC50 = 4.8 nM 8.32
CHEMBL5172205 EC50 = 9.0 nM 8.05
CHEMBL5209093 EC50 = 14.7 nM 7.83
CHEMBL5180999 EC50 = 15.9 nM 7.80
CHEMBL5188839 EC50 = 17.6 nM 7.75
CHEMBL5207186 EC50 = 24.2 nM 7.62
CHEMBL5186156 EC50 = 26.6 nM 7.58
CHEMBL4163070 EC50 = 29.8 nM 7.53
CHEMBL1544 LIOTHYRONINE EC50 = 29.7 nM 7.53
CHEMBL2338329 ROXADUSTAT EC50 = 32.7 nM 7.49
CHEMBL5179829 EC50 = 32.8 nM 7.48
CHEMBL5191021 EC50 = 36.4 nM 7.44
CHEMBL5171808 EC50 = 39.0 nM 7.41
CHEMBL5207720 EC50 = 45.1 nM 7.35
CHEMBL5193673 EC50 = 53.0 nM 7.28
CHEMBL5180738 EC50 = 56.8 nM 7.25
CHEMBL5207967 EC50 = 69.1 nM 7.16
CHEMBL5191155 EC50 = 90.7 nM 7.04
CHEMBL5206520 EC50 = 270.4 nM 6.57
CHEMBL5171818 EC50 = 302.5 nM 6.52
CHEMBL3261331 RESMETIROM EC50 = 2735.0 nM 5.56
CHEMBL5200322 Activity - -
CHEMBL5178993 Activity - -
CHEMBL5178553 Activity - -
CHEMBL5181604 Activity - -
CHEMBL5204264 Activity - -
CHEMBL5205719 Activity - -
CHEMBL5173831 Activity - -