Assay Detail
Functional
CHEMBL5209609
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745864)
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Membrane potential based transport assay for SLC1A3 using HEK293 JumpIn SLC1A3 OE cells
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 4.81 | 4.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL274323 | ASPARTIC ACID | 3.0 | 1 | 4.82 |
| CHEMBL575060 | GLUTAMIC ACID | 3.0 | 1 | 4.80 |
| CHEMBL863 | CYSTEINE | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL274323 | ASPARTIC ACID | EC50 | = | 15000.0 | nM | 4.82 |
| CHEMBL575060 | GLUTAMIC ACID | EC50 | = | 16000.0 | nM | 4.80 |
| CHEMBL863 | CYSTEINE | EC50 | = | 376000.0 | nM | - |