Assay Detail
Functional
CHEMBL5209616
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by L-glutamate uptake in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745862)
6
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Impedance-Based Phenotypic Readout of Transporter Function: A Case for Glutamate Transporters
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | - | - |
| IC50 | 2 | 5.95 | 6.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1257519 | — | — | 1 | 6.70 |
| CHEMBL474133 | — | — | 1 | 5.20 |
| CHEMBL575060 | GLUTAMIC ACID | 3.0 | 1 | - |
| CHEMBL76232 | D-GLUTAMATE | — | 1 | - |
| CHEMBL274323 | ASPARTIC ACID | 3.0 | 1 | - |
| CHEMBL29757 | D-ASPARTATE | 2.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1257519 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL474133 | — | IC50 | = | 6300.0 | nM | 5.20 |
| CHEMBL575060 | GLUTAMIC ACID | EC50 | = | 398000.0 | nM | - |
| CHEMBL76232 | D-GLUTAMATE | EC50 | > | 1000000.0 | nM | - |
| CHEMBL274323 | ASPARTIC ACID | EC50 | = | 398000.0 | nM | - |
| CHEMBL29757 | D-ASPARTATE | EC50 | = | 398000.0 | nM | - |