Assay Detail
Functional
CHEMBL5209935
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Affinity Phenotypic Cellular interaction (CellTiter-Glo® Luminescent Cell Viability Assay (ZR-75-1; breast cancer cells)) EUB0000645a AKT1
1
Total Activities
1
Compounds Tested
1
Activity Types
3
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Category | Affinity phenotypic cellular assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
RAC-alpha serine/threonine-protein kinase (CHEMBL4282) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Affinity Phenotypic Cellular Literature for EUbOPEN Chemogenomics Library wave 3
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 8.30 | 8.30 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Mode of action | Mode of action | - | — | Covalent-allosteric inhibitor |
| Protein family | Protein family | - | — | Protein Kinase |
| Recommended concentration | Recommended concentration | 1.0 | uM | — |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4795065 | Borussertib | — | 1 | 8.30 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4795065 | Borussertib | EC50 | = | 5.0 | nM | 8.30 |