Assay Detail
Binding
CHEMBL5210304
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Affinity On-target Cellular interaction (EGFR-phosphorylation in A431 cells) EUB0000596aBDA EGFR
1
Total Activities
1
Compounds Tested
1
Activity Types
3
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | Affinity on-target cellular assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Affinity On-target Cellular Literature for EUbOPEN Chemogenomics Library wave 3
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 7.89 | 7.89 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Mode of action | Mode of action | - | — | Covalent inhibitor |
| Protein family | Protein family | - | — | Protein Kinase |
| Recommended concentration | Recommended concentration | 100.0 | nM | — |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2105712 | AFATINIB DIMALEATE | 4.0 | 1 | 7.89 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2105712 | AFATINIB DIMALEATE | EC50 | = | 13.0 | nM | 7.89 |