Assay Detail
Binding
CHEMBL5216110
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to recombinant full length N-terminal hexahistidine-tagged human FTO expressed in Escherichia coli BL21 by competition-based NMR binding assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Alpha-ketoglutarate-dependent dioxygenase FTO (CHEMBL2331065) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-Based Design of Selective Fat Mass and Obesity Associated Protein (FTO) Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 4 | 5.99 | 6.47 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5219846 | — | — | 1 | 6.47 |
| CHEMBL5219336 | — | — | 1 | 6.17 |
| CHEMBL5218636 | — | — | 1 | 5.92 |
| CHEMBL5220246 | — | — | 1 | 5.41 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5219846 | — | Kd | = | 340.0 | nM | 6.47 |
| CHEMBL5219336 | — | Kd | = | 670.0 | nM | 6.17 |
| CHEMBL5218636 | — | Kd | = | 1200.0 | nM | 5.92 |
| CHEMBL5220246 | — | Kd | = | 3900.0 | nM | 5.41 |