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Assay Detail

CHEMBL5216112

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD4 (unknown origin) expressed in Escherichia coli BL21 (DE3) using [Lys(Ac)5/8/12/16]-histone H4 (1 to 21residues)-GGK as substrate incubated for 30 mins by TR-FRET assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel BRD4 Ligand Scaffolds by Automated Navigation of the Fragment Chemical Space.
Piticchio SG, Martínez-Cartró M, Scaffidi S, Rachman M, Rodriguez-Arevalo S, Sanchez-Arfelis A, Escolano C, Picaud S, Krojer T, Filippakopoulos P, von Delft F, Galdeano C, Barril X.
J Med Chem (2021) 64 : 17887 -17900
PubMed 34898210 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 3.96 4.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5218482 1 4.58
CHEMBL5219547 1 4.52
CHEMBL197512 1 4.14
CHEMBL5220412 1 4.10
CHEMBL5220338 1 4.07
CHEMBL2431088 1 4.04
CHEMBL5220563 1 3.72
CHEMBL5218934 1 3.31
CHEMBL5219925 1 3.19
CHEMBL5219301 1 -
CHEMBL5220571 1 -
CHEMBL5220909 1 -
CHEMBL5220081 1 -
CHEMBL1536867 1 -
CHEMBL5219185 1 -
CHEMBL5220065 1 -
CHEMBL5218493 1 -
CHEMBL5219610 1 -
CHEMBL5218610 1 -
CHEMBL5219368 1 -
CHEMBL5219549 1 -
CHEMBL5219295 1 -
CHEMBL5220722 1 -
CHEMBL4470721 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5218482 IC50 = 26000.0 nM 4.58
CHEMBL5219547 IC50 = 30000.0 nM 4.52
CHEMBL197512 IC50 = 72000.0 nM 4.14
CHEMBL5220412 IC50 = 79000.0 nM 4.10
CHEMBL5220338 IC50 = 86000.0 nM 4.07
CHEMBL2431088 IC50 = 91000.0 nM 4.04
CHEMBL5220563 IC50 = 191000.0 nM 3.72
CHEMBL5218934 IC50 = 491000.0 nM 3.31
CHEMBL5219925 IC50 = 644000.0 nM 3.19
CHEMBL5219301 IC50 - -
CHEMBL5220571 IC50 - -
CHEMBL5220909 IC50 - -
CHEMBL5220081 IC50 - -
CHEMBL1536867 IC50 - -
CHEMBL5219185 IC50 - -
CHEMBL5220065 IC50 - -
CHEMBL5218493 IC50 - -
CHEMBL5219610 IC50 - -
CHEMBL5218610 IC50 - -
CHEMBL5219368 IC50 - -
CHEMBL5219549 IC50 - -
CHEMBL5219295 IC50 - -
CHEMBL5220722 IC50 - -
CHEMBL4470721 IC50 - -