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Assay Detail

CHEMBL5223240

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant SARS-CoV-2 Main protease expressed in Escherichia coli using MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 as substrate preincubated for 10 mins followed by substrate addition and measured every 30 sec for 10 mins by FRET assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

A review of the latest research on M<sup>pro</sup> targeting SARS-COV inhibitors.
Yang H, Yang J.
RSC Med Chem (2021) 12 : 1026 -1036
PubMed 34355175 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.35 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4764751 1 7.40
CHEMBL4796938 1 7.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4764751 IC50 = 40.0 nM 7.40
CHEMBL4796938 IC50 = 50.0 nM 7.30