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Assay Detail

CHEMBL5227868

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at KOR (unknown origin) expressed in CHO cell membranes assessed as stimulation of [35S]GTPgammaS binding measured after 60 mins by liquid scintillation counting analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery, Structure-Activity Relationship, and Mechanistic Studies of 1-((3<i>R</i>,4<i>S</i>)-3-((Dimethylamino)methyl)-4-hydroxy-4-(3-methoxyphenyl)piperidin-1-yl)-2-(2,4,5-trifluorophenyl)ethan-1-one as a Novel Potent Analgesic.
Huang H, Li X, Xie P, Li X, Xu X, Qian Y, Yuan C, Meng X, Chai J, Chen J, Liu J, Wang W, Li W, Wang Y, Fu W, Liu J.
J Med Chem (2021) 64 : 9458 -9483
PubMed 34152138 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 6.85 8.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL290543 1 8.55
CHEMBL5273356 1 7.12
CHEMBL5287792 1 6.98
CHEMBL5277326 1 6.57
CHEMBL5270915 1 6.46
CHEMBL5287134 1 6.30
CHEMBL5279890 1 5.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL290543 EC50 = 2.8 nM 8.55
CHEMBL5273356 EC50 = 75.4 nM 7.12
CHEMBL5287792 EC50 = 105.4 nM 6.98
CHEMBL5277326 EC50 = 267.3 nM 6.57
CHEMBL5270915 EC50 = 342.8 nM 6.46
CHEMBL5287134 EC50 = 500.2 nM 6.30
CHEMBL5279890 EC50 = 1032.0 nM 5.99