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Assay Detail

CHEMBL5229116

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli AlkB using 15-mer m1A-ssDNA as substrate incubated for 10 mins by HPLC analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Alpha-ketoglutarate-dependent dioxygenase AlkB (CHEMBL5291506)
Type SINGLE PROTEIN
Organism Escherichia coli (strain K12)

Publication

Inhibition of AlkB Nucleic Acid Demethylases: Promising New Epigenetic Targets.
Perry GS, Das M, Woon ECY.
J Med Chem (2021) 64 : 16974 -17003
PubMed 34792334 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.47 4.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5173876 1 4.47
CHEMBL90852 N-OXALYLGLYCINE 1 -
CHEMBL5274121 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5173876 IC50 = 33500.0 nM 4.47
CHEMBL90852 N-OXALYLGLYCINE IC50 = 108300.0 nM -
CHEMBL5274121 IC50 = 168800.0 nM -