Assay Detail
Binding
CHEMBL5229162
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal 31 residues truncated FTO (unknown origin) demethylase activity using m6A containing ssRNA as substrate incubated for 1 hr by LC-MS analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Alpha-ketoglutarate-dependent dioxygenase FTO (CHEMBL2331065) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibition of AlkB Nucleic Acid Demethylases: Promising New Epigenetic Targets.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.39 | 5.76 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5282054 | — | — | 1 | 5.76 |
| CHEMBL5280448 | — | — | 1 | 5.65 |
| CHEMBL1057 | FLUORESCEIN | 4.0 | 1 | 5.49 |
| CHEMBL1200433 | — | — | 1 | 5.44 |
| CHEMBL5271944 | — | — | 1 | 5.38 |
| CHEMBL5280899 | — | — | 1 | 5.35 |
| CHEMBL5279808 | — | — | 1 | 5.32 |
| CHEMBL5284644 | — | — | 1 | 5.18 |
| CHEMBL5285693 | — | — | 1 | 5.18 |
| CHEMBL5268591 | — | — | 1 | 5.18 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5282054 | — | IC50 | = | 1720.0 | nM | 5.76 |
| CHEMBL5280448 | — | IC50 | = | 2240.0 | nM | 5.65 |
| CHEMBL1057 | FLUORESCEIN | IC50 | = | 3230.0 | nM | 5.49 |
| CHEMBL1200433 | — | IC50 | = | 3650.0 | nM | 5.44 |
| CHEMBL5271944 | — | IC50 | = | 4120.0 | nM | 5.38 |
| CHEMBL5280899 | — | IC50 | = | 4490.0 | nM | 5.35 |
| CHEMBL5279808 | — | IC50 | = | 4840.0 | nM | 5.32 |
| CHEMBL5284644 | — | IC50 | = | 6550.0 | nM | 5.18 |
| CHEMBL5285693 | — | IC50 | = | 6600.0 | nM | 5.18 |
| CHEMBL5268591 | — | IC50 | = | 6650.0 | nM | 5.18 |