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Assay Detail

CHEMBL5229172

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FTO (unknown origin)-mediated demethylation activity using 15-mer ssRNA with CUUGUCA(m6A)CAGCAGA sequence as substrate by reverse phase high-performance liquid chromatography
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-ketoglutarate-dependent dioxygenase FTO (CHEMBL2331065)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibition of AlkB Nucleic Acid Demethylases: Promising New Epigenetic Targets.
Perry GS, Das M, Woon ECY.
J Med Chem (2021) 64 : 16974 -17003
PubMed 34792334 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.56 4.56

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL235934 CLAUSINE E 1 4.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL235934 CLAUSINE E IC50 = 27790.0 nM 4.56