Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5229864

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His tagged SARS-CoV-2 PLpro using Z-RLRGG-AMC as substrate assessed as substrate cleavage preincubated for 60 mins followed by substrate addition and measured every 3 mins for 72 mins by fluorescence based assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Identification of Natural Products Inhibiting SARS-CoV-2 by Targeting Viral Proteases: A Combined in Silico and in Vitro Approach.
Wasilewicz A, Kirchweger B, Bojkova D, Abi Saad MJ, Langeder J, Bütikofer M, Adelsberger S, Grienke U, Cinatl J, Petermann O, Scapozza L, Orts J, Kirchmair J, Rabenau HF, Rollinger JM.
J Nat Prod (2023) 86 : 264 -275
PubMed 36651644 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.36 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL549695 1 6.10
CHEMBL403516 ACETYLSHIKONIN 1 4.61

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL549695 IC50 = 800.0 nM 6.10
CHEMBL403516 ACETYLSHIKONIN IC50 = 24300.0 nM 4.61