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Assay Detail

CHEMBL5231345

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Binding
Inhibition of Escherichia coli DH5alpha FabI in the presence of NADH using crotonyl CoA as substrate by measuring NADH consumption rate
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (CHEMBL1857)
Type SINGLE PROTEIN
Organism Escherichia coli K-12

Publication

FabI (enoyl acyl carrier protein reductase) - A potential broad spectrum therapeutic target and its inhibitors.
Rana P, Ghouse SM, Akunuri R, Madhavi YV, Chopra S, Nanduri S.
Eur J Med Chem (2020) 208 : 112757 -112757
PubMed 32883635 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.07 7.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2178284 1 7.24
CHEMBL5274978 1 6.47
CHEMBL5276897 1 6.40
CHEMBL463175 CEPHALOCHROMIN 1 5.75
CHEMBL5277493 1 4.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2178284 IC50 = 58.0 nM 7.24
CHEMBL5274978 IC50 = 340.0 nM 6.47
CHEMBL5276897 IC50 = 400.0 nM 6.40
CHEMBL463175 CEPHALOCHROMIN IC50 = 1800.0 nM 5.75
CHEMBL5277493 IC50 = 33200.0 nM 4.48