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Assay Detail

CHEMBL5233540

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant STAT3 (unknown origin) expressed in baculovirus in Sf9 insect cells assessed as reduction in DNA binding activity with NIH3T3 nuclear extract incubated for 30 mins by electrophoretic mobility shift assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Signal transducer and activator of transcription 3 (CHEMBL4026)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Revisiting signal transducer and activator of transcription 3 (STAT3) as an anticancer target and its inhibitor discovery: Where are we and where should we go?
Huang Q, Zhong Y, Dong H, Zheng Q, Shi S, Zhu K, Qu X, Hu W, Zhang X, Wang Y.
Eur J Med Chem (2020) 187 : 111922 -111922
PubMed 31810784 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 4.59 5.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1829876 1 5.17
CHEMBL2172015 1 4.48
CHEMBL1829786 1 4.46
CHEMBL2172018 1 4.24
CHEMBL5276064 1 -
CHEMBL5280162 1 -
CHEMBL3696067 1 -
CHEMBL2431982 1 -
CHEMBL5267613 1 -
CHEMBL5279594 1 -
CHEMBL5266557 1 -
CHEMBL2431981 1 -
CHEMBL5277946 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1829876 IC50 = 6800.0 nM 5.17
CHEMBL2172015 IC50 = 33000.0 nM 4.48
CHEMBL1829786 IC50 = 35000.0 nM 4.46
CHEMBL2172018 IC50 = 58000.0 nM 4.24
CHEMBL5276064 IC50 = 182000.0 nM -
CHEMBL5280162 IC50 = 217000.0 nM -
CHEMBL3696067 IC50 = 255000.0 nM -
CHEMBL2431982 IC50 > 300000.0 nM -
CHEMBL5267613 IC50 = 292000.0 nM -
CHEMBL5279594 IC50 = 115000.0 nM -
CHEMBL5266557 IC50 = 194000.0 nM -
CHEMBL2431981 IC50 > 300000.0 nM -
CHEMBL5277946 IC50 = 235000.0 nM -