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Assay Detail

CHEMBL5236940

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CDK9/Cyclin-T1 (unknown origin) assessed as reduction in substrate peptide phosphorylation using DYRKtide peptide as substrate preincubated for 12 mins followed by ATP addition and measured for 45 mins by mobility shift assay
23
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK9/cyclin T1 (CHEMBL2111389)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of PF-06873600, a CDK2/4/6 Inhibitor for the Treatment of Cancer.
Freeman-Cook KD, Hoffman RL, Behenna DC, Boras B, Carelli J, Diehl W, Ferre RA, He YA, Hui A, Huang B, Huser N, Jones R, Kephart SE, Lapek J, McTigue M, Miller N, Murray BW, Nagata A, Nguyen L, Niessen S, Ninkovic S, O'Doherty I, Ornelas MA, Solowiej J, Sutton SC, Tran K, Tseng E, Visswanathan R, Xu M, Zehnder L, Zhang Q, Zhang C, Dann S.
J Med Chem (2021) 64 : 9056 -9077
PubMed 34110834 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 23 7.48 8.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5276905 1 8.21
CHEMBL5281860 1 8.10
CHEMBL5269336 1 8.10
CHEMBL5280928 1 8.02
CHEMBL5269912 1 7.82
CHEMBL5287843 1 7.79
CHEMBL5277190 2 7.74
CHEMBL4446357 EBVACICLIB 2.0 1 7.71
CHEMBL4752291 1 7.52
CHEMBL5278622 1 7.50
CHEMBL5271753 1 7.43
CHEMBL5272948 1 7.41
CHEMBL5271882 1 7.38
CHEMBL4796501 1 7.27
CHEMBL5283828 1 6.96
CHEMBL5285163 1 6.88
CHEMBL5278015 1 6.79
CHEMBL189963 PALBOCICLIB 4.0 1 6.70
CHEMBL5281770 1 6.67
CHEMBL5275378 1 -
CHEMBL5266837 1 -
CHEMBL5288078 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5276905 Ki = 6.2 nM 8.21
CHEMBL5281860 Ki = 8.0 nM 8.10
CHEMBL5269336 Ki = 8.0 nM 8.10
CHEMBL5280928 Ki = 9.6 nM 8.02
CHEMBL5269912 Ki = 15.0 nM 7.82
CHEMBL5287843 Ki = 16.3 nM 7.79
CHEMBL5277190 Ki = 18.4 nM 7.74
CHEMBL4446357 EBVACICLIB Ki = 19.6 nM 7.71
CHEMBL5277190 Ki = 22.7 nM 7.64
CHEMBL4752291 Ki = 30.0 nM 7.52
CHEMBL5278622 Ki = 31.4 nM 7.50
CHEMBL5271753 Ki = 36.8 nM 7.43
CHEMBL5272948 Ki = 39.1 nM 7.41
CHEMBL5271882 Ki = 41.3 nM 7.38
CHEMBL4796501 Ki = 53.2 nM 7.27
CHEMBL5283828 Ki = 109.0 nM 6.96
CHEMBL5285163 Ki = 132.0 nM 6.88
CHEMBL5278015 Ki = 163.0 nM 6.79
CHEMBL189963 PALBOCICLIB Ki = 201.0 nM 6.70
CHEMBL5281770 Ki = 214.0 nM 6.67
CHEMBL5275378 Ki > 1200.0 nM -
CHEMBL5266837 Ki > 1400.0 nM -
CHEMBL5288078 Ki > 1200.0 nM -