Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5239394

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant N-terminal GST-tagged human DLK (9 to 111 residues) expressed in Sf21 cells assessed as reduction in substrate phosphorylation using MKK4 and ATP as substrate incubated for 1.5 hrs by HTRF analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase kinase kinase 12 (CHEMBL1908389)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Potent and Selective Dual Leucine Zipper Kinase/Leucine Zipper-Bearing Kinase Inhibitors with Neuroprotective Properties in In Vitro and In Vivo Models of Amyotrophic Lateral Sclerosis.
Craig RA, Fox BM, Hu C, Lexa KW, Osipov M, Thottumkara AP, Larhammar M, Miyamoto T, Rana A, Kane LA, Yulyaningsih E, Solanoy H, Nguyen H, Chau R, Earr T, Kajiwara Y, Fleck D, Lucas A, Haddick PCG, Takahashi RH, Tong V, Wang J, Canet MJ, Poda SB, Scearce-Levie K, Srivastava A, Sweeney ZK, Xu M, Zhang R, He J, Lei Y, Zhuo Z, de Vicente J.
J Med Chem (2022) 65 : 16290 -16312
PubMed 36469401 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 7.88 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5272522 1 8.70
CHEMBL5286166 1 8.70
CHEMBL5283101 1 8.52
CHEMBL5281494 1 8.52
CHEMBL5288706 1 8.52
CHEMBL5268969 1 8.40
CHEMBL5271665 1 8.40
CHEMBL5271972 1 8.40
CHEMBL5270353 1 7.92
CHEMBL5276415 1 7.89
CHEMBL5268950 1 7.89
CHEMBL5276337 1 7.89
CHEMBL5277276 1 7.85
CHEMBL5284890 1 7.77
CHEMBL5278185 1 7.62
CHEMBL5269484 1 7.55
CHEMBL5278476 1 7.33
CHEMBL5273015 1 7.28
CHEMBL5284496 1 7.28
CHEMBL5269792 1 7.21
CHEMBL5277751 1 7.11
CHEMBL5281886 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5272522 IC50 = 2.0 nM 8.70
CHEMBL5286166 IC50 = 2.0 nM 8.70
CHEMBL5283101 IC50 = 3.0 nM 8.52
CHEMBL5281494 IC50 = 3.0 nM 8.52
CHEMBL5288706 IC50 = 3.0 nM 8.52
CHEMBL5268969 IC50 = 4.0 nM 8.40
CHEMBL5271665 IC50 = 4.0 nM 8.40
CHEMBL5271972 IC50 = 4.0 nM 8.40
CHEMBL5270353 IC50 = 12.0 nM 7.92
CHEMBL5276415 IC50 = 13.0 nM 7.89
CHEMBL5268950 IC50 = 13.0 nM 7.89
CHEMBL5276337 IC50 = 13.0 nM 7.89
CHEMBL5277276 IC50 = 14.0 nM 7.85
CHEMBL5284890 IC50 = 17.0 nM 7.77
CHEMBL5278185 IC50 = 24.0 nM 7.62
CHEMBL5269484 IC50 = 28.0 nM 7.55
CHEMBL5278476 IC50 = 47.0 nM 7.33
CHEMBL5273015 IC50 = 53.0 nM 7.28
CHEMBL5284496 IC50 = 53.0 nM 7.28
CHEMBL5269792 IC50 = 61.0 nM 7.21
CHEMBL5277751 IC50 = 77.0 nM 7.11
CHEMBL5281886 IC50 = 201.0 nM 6.70