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Assay Detail

CHEMBL5239396

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DLK expressed in HEK cells assessed as reduction in phospho-c-Jun level preincubated for 45 mins followed by doxycycline stimulation and measured after 24 hrs by MSD ELISA
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase kinase kinase 12 (CHEMBL1908389)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Potent and Selective Dual Leucine Zipper Kinase/Leucine Zipper-Bearing Kinase Inhibitors with Neuroprotective Properties in In Vitro and In Vivo Models of Amyotrophic Lateral Sclerosis.
Craig RA, Fox BM, Hu C, Lexa KW, Osipov M, Thottumkara AP, Larhammar M, Miyamoto T, Rana A, Kane LA, Yulyaningsih E, Solanoy H, Nguyen H, Chau R, Earr T, Kajiwara Y, Fleck D, Lucas A, Haddick PCG, Takahashi RH, Tong V, Wang J, Canet MJ, Poda SB, Scearce-Levie K, Srivastava A, Sweeney ZK, Xu M, Zhang R, He J, Lei Y, Zhuo Z, de Vicente J.
J Med Chem (2022) 65 : 16290 -16312
PubMed 36469401 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 7.06 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5272522 1 8.40
CHEMBL5286166 1 8.00
CHEMBL5271665 1 7.72
CHEMBL5271972 1 7.57
CHEMBL5288706 1 7.44
CHEMBL5283101 1 7.35
CHEMBL5276415 1 7.29
CHEMBL5268969 1 7.24
CHEMBL5270353 1 7.17
CHEMBL5269792 1 7.11
CHEMBL5284890 1 7.06
CHEMBL5276337 1 6.97
CHEMBL5268950 1 6.84
CHEMBL5273015 1 6.77
CHEMBL5278185 1 6.74
CHEMBL5277276 1 6.73
CHEMBL5269484 1 6.67
CHEMBL5277751 1 6.59
CHEMBL5284496 1 6.49
CHEMBL5278476 1 6.04
CHEMBL5281886 1 5.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5272522 EC50 = 4.0 nM 8.40
CHEMBL5286166 EC50 = 10.0 nM 8.00
CHEMBL5271665 EC50 = 19.0 nM 7.72
CHEMBL5271972 EC50 = 27.0 nM 7.57
CHEMBL5288706 EC50 = 36.0 nM 7.44
CHEMBL5283101 EC50 = 45.0 nM 7.35
CHEMBL5276415 EC50 = 51.0 nM 7.29
CHEMBL5268969 EC50 = 58.0 nM 7.24
CHEMBL5270353 EC50 = 67.0 nM 7.17
CHEMBL5269792 EC50 = 77.0 nM 7.11
CHEMBL5284890 EC50 = 88.0 nM 7.06
CHEMBL5276337 EC50 = 107.0 nM 6.97
CHEMBL5268950 EC50 = 143.0 nM 6.84
CHEMBL5273015 EC50 = 171.0 nM 6.77
CHEMBL5278185 EC50 = 183.0 nM 6.74
CHEMBL5277276 EC50 = 185.0 nM 6.73
CHEMBL5269484 EC50 = 212.0 nM 6.67
CHEMBL5277751 EC50 = 258.0 nM 6.59
CHEMBL5284496 EC50 = 323.0 nM 6.49
CHEMBL5278476 EC50 = 902.0 nM 6.04
CHEMBL5281886 EC50 = 1072.0 nM 5.97