Assay Detail
Binding
CHEMBL5239841
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3H]N-methylscopolamine from human muscarinic M4 receptor expressed in human HEK293T cell membranes by radioligand competition binding based analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293T |
| Subcellular | Cell membrane |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL1821) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis, Characterization, and Application of Muscarinergic M<sub>3</sub> Receptor Ligands Linked to Fluorescent Dyes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 10 | 8.78 | 9.25 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5289601 | — | — | 1 | 9.25 |
| CHEMBL5289667 | — | — | 1 | 9.07 |
| CHEMBL5271406 | — | — | 1 | 9.06 |
| CHEMBL5273907 | — | — | 1 | 9.05 |
| CHEMBL5285607 | — | — | 1 | 8.85 |
| CHEMBL5289083 | — | — | 1 | 8.77 |
| CHEMBL5268503 | — | — | 1 | 8.59 |
| CHEMBL5283759 | — | — | 1 | 8.48 |
| CHEMBL5267836 | — | — | 1 | 8.42 |
| CHEMBL5277670 | — | — | 1 | 8.27 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5289601 | — | Ki | = | 0.56 | nM | 9.25 |
| CHEMBL5289667 | — | Ki | = | 0.86 | nM | 9.07 |
| CHEMBL5271406 | — | Ki | = | 0.87 | nM | 9.06 |
| CHEMBL5273907 | — | Ki | = | 0.9 | nM | 9.05 |
| CHEMBL5285607 | — | Ki | = | 1.4 | nM | 8.85 |
| CHEMBL5289083 | — | Ki | = | 1.7 | nM | 8.77 |
| CHEMBL5268503 | — | Ki | = | 2.6 | nM | 8.59 |
| CHEMBL5283759 | — | Ki | = | 3.3 | nM | 8.48 |
| CHEMBL5267836 | — | Ki | = | 3.8 | nM | 8.42 |
| CHEMBL5277670 | — | Ki | = | 5.4 | nM | 8.27 |