Assay Detail
Binding
CHEMBL5239848
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to human muscarinic M3 receptor expressed in human HEK293T cells cells co-expressing beta-arrestin2 assessed as inhibition of carbachol-induced beta-arrestin recruitment preincubated for 30 mins followed by carbachol stimulation and measured after 90 mins by PathHunter assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis, Characterization, and Application of Muscarinergic M<sub>3</sub> Receptor Ligands Linked to Fluorescent Dyes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.36 | 6.37 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5285607 | — | — | 1 | 6.37 |
| CHEMBL5271406 | — | — | 1 | 6.35 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5285607 | — | IC50 | = | 430.0 | nM | 6.37 |
| CHEMBL5271406 | — | IC50 | = | 450.0 | nM | 6.35 |