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Assay Detail

CHEMBL5247339

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS CoV-2 Main protease using MCA-AVLQSGFR-Lys(DNP)-Lys-NH2 peptide as substrate by fluorescence based analysis
31
Total Activities
29
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Perspectives on SARS-CoV-2 Main Protease Inhibitors.
Gao K, Wang R, Chen J, Tepe JJ, Huang F, Wei GW.
J Med Chem (2021) 64 : 16922 -16955
PubMed 34798775 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 6.51 7.13
Ki 2 7.31 7.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5266355 2 7.51
CHEMBL5179454 2 7.11
CHEMBL468553 1 7.09
CHEMBL3655715 1 6.89
CHEMBL4079667 1 6.89
CHEMBL5283181 1 6.82
CHEMBL5267356 1 6.82
CHEMBL99313 1 6.77
CHEMBL1435753 1 6.64
CHEMBL4779667 1 6.62
CHEMBL5195364 1 6.58
CHEMBL5270064 1 6.48
CHEMBL1336959 1 6.48
CHEMBL4552531 1 6.46
CHEMBL4060715 1 6.44
CHEMBL1394251 1 6.42
CHEMBL98386 1 6.41
CHEMBL4068618 1 6.39
CHEMBL5277713 1 6.39
CHEMBL45016 1 6.39
CHEMBL3219171 1 6.32
CHEMBL45152 1 6.31
CHEMBL51085 EBSELEN 3.0 1 6.28
CHEMBL2391913 1 6.20
CHEMBL4783878 1 6.19
CHEMBL297304 1 6.18
CHEMBL5280783 1 6.14
CHEMBL1710047 1 6.11
CHEMBL5274064 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5266355 Ki = 31.0 nM 7.51
CHEMBL5266355 IC50 = 74.0 nM 7.13
CHEMBL5179454 Ki = 78.0 nM 7.11
CHEMBL468553 IC50 = 81.0 nM 7.09
CHEMBL5179454 IC50 = 110.0 nM 6.96
CHEMBL4079667 IC50 = 130.0 nM 6.89
CHEMBL3655715 IC50 = 130.0 nM 6.89
CHEMBL5267356 IC50 = 151.0 nM 6.82
CHEMBL5283181 IC50 = 150.0 nM 6.82
CHEMBL99313 IC50 = 170.0 nM 6.77
CHEMBL1435753 IC50 = 230.0 nM 6.64
CHEMBL4779667 IC50 = 240.0 nM 6.62
CHEMBL5195364 IC50 = 260.0 nM 6.58
CHEMBL1336959 IC50 = 330.0 nM 6.48
CHEMBL5270064 IC50 = 330.0 nM 6.48
CHEMBL4552531 IC50 = 350.0 nM 6.46
CHEMBL4060715 IC50 = 360.0 nM 6.44
CHEMBL1394251 IC50 = 380.0 nM 6.42
CHEMBL98386 IC50 = 390.0 nM 6.41
CHEMBL4068618 IC50 = 410.0 nM 6.39
CHEMBL45016 IC50 = 410.0 nM 6.39
CHEMBL5277713 IC50 = 410.0 nM 6.39
CHEMBL3219171 IC50 = 480.0 nM 6.32
CHEMBL45152 IC50 = 490.0 nM 6.31
CHEMBL51085 EBSELEN IC50 = 520.0 nM 6.28
CHEMBL2391913 IC50 = 630.0 nM 6.20
CHEMBL4783878 IC50 = 650.0 nM 6.19
CHEMBL297304 IC50 = 660.0 nM 6.18
CHEMBL5280783 IC50 = 730.0 nM 6.14
CHEMBL1710047 IC50 = 780.0 nM 6.11
CHEMBL5274064 IC50 = 910.0 nM 6.04