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Assay Detail

CHEMBL5248749

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human URAT1 mediated 14C-uric acid uptake expressed in HEK293 cells using 14C-uric acid as substrate incubated for 30 mins by liquid scintillation counting analysis
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Solute carrier family 22 member 12 (CHEMBL6120)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and activity evaluation of novel lesinurad analogues containing thienopyrimidinone or pyridine substructure as human urate transporter 1 inhibitors.
Zhang J, Dong Y, Gao S, Zhang X, Liao H, Shi X, Zhang Z, Zhao T, Liang R, Qi D, Wu T, Pang J, Liu X, Zhan P.
Eur J Med Chem (2022) 244 : 114816 -114816
PubMed 36219903 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 4.97 5.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5271398 1 5.49
CHEMBL4756866 1 5.38
CHEMBL5277301 1 5.14
CHEMBL5287151 1 5.12
CHEMBL5284974 1 5.12
CHEMBL5289202 1 5.10
CHEMBL2105720 LESINURAD 4.0 1 5.03
CHEMBL5269373 1 5.03
CHEMBL5289441 1 5.01
CHEMBL5278085 1 5.01
CHEMBL5273217 1 5.01
CHEMBL5279672 1 5.01
CHEMBL5277066 1 5.00
CHEMBL5268478 1 5.00
CHEMBL5290258 1 4.99
CHEMBL5282615 1 4.96
CHEMBL5267727 1 4.95
CHEMBL5285786 1 4.95
CHEMBL5267010 1 4.94
CHEMBL5291373 1 4.94
CHEMBL5285533 1 4.94
CHEMBL5268476 1 4.93
CHEMBL5282586 1 4.92
CHEMBL5290341 1 4.92
CHEMBL5266247 1 4.91
CHEMBL5272958 1 4.87
CHEMBL5278304 1 4.86
CHEMBL5272326 1 4.81
CHEMBL5282047 1 4.80
CHEMBL5269609 1 4.79

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5271398 IC50 = 3270.0 nM 5.49
CHEMBL4756866 IC50 = 4170.0 nM 5.38
CHEMBL5277301 IC50 = 7310.0 nM 5.14
CHEMBL5287151 IC50 = 7680.0 nM 5.12
CHEMBL5284974 IC50 = 7560.0 nM 5.12
CHEMBL5289202 IC50 = 7900.0 nM 5.10
CHEMBL2105720 LESINURAD IC50 = 9380.0 nM 5.03
CHEMBL5269373 IC50 = 9250.0 nM 5.03
CHEMBL5289441 IC50 = 9820.0 nM 5.01
CHEMBL5278085 IC50 = 9700.0 nM 5.01
CHEMBL5273217 IC50 = 9790.0 nM 5.01
CHEMBL5279672 IC50 = 9720.0 nM 5.01
CHEMBL5277066 IC50 = 9920.0 nM 5.00
CHEMBL5268478 IC50 = 10090.0 nM 5.00
CHEMBL5290258 IC50 = 10310.0 nM 4.99
CHEMBL5282615 IC50 = 11030.0 nM 4.96
CHEMBL5267727 IC50 = 11120.0 nM 4.95
CHEMBL5285786 IC50 = 11130.0 nM 4.95
CHEMBL5267010 IC50 = 11380.0 nM 4.94
CHEMBL5291373 IC50 = 11400.0 nM 4.94
CHEMBL5285533 IC50 = 11540.0 nM 4.94
CHEMBL5268476 IC50 = 11650.0 nM 4.93
CHEMBL5282586 IC50 = 11930.0 nM 4.92
CHEMBL5290341 IC50 = 12070.0 nM 4.92
CHEMBL5266247 IC50 = 12190.0 nM 4.91
CHEMBL5272958 IC50 = 13460.0 nM 4.87
CHEMBL5278304 IC50 = 13670.0 nM 4.86
CHEMBL5272326 IC50 = 15350.0 nM 4.81
CHEMBL5282047 IC50 = 15790.0 nM 4.80
CHEMBL5269609 IC50 = 16250.0 nM 4.79
CHEMBL5284756 IC50 = 17240.0 nM 4.76
CHEMBL5287955 IC50 = 18740.0 nM 4.73
CHEMBL5270485 IC50 = 20320.0 nM 4.69