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Assay Detail

CHEMBL5250948

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of hexa His-tagged DYRK2 (72 to 479 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as substrate phosphorylation using 4E-BP1 as substrate in presence of [gamma33-P]ATP incubated for 2 hrs by scintillation counter analysis
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of Potent DYRK2 Inhibitors with High Selectivity, Great Solubility, and Excellent Safety Properties for the Treatment of Prostate Cancer.
Yuan K, Shen H, Zheng M, Xia F, Li Q, Chen W, Ji M, Yang H, Zhuang X, Cai Z, Min W, Wang X, Xiao Y, Yang P.
J Med Chem (2023) 66 : 4215 -4230
PubMed 36800260 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.91 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5289179 1 9.22
CHEMBL5286478 1 8.40
CHEMBL5272300 1 7.82
CHEMBL5278493 1 7.80
CHEMBL5277818 1 7.66
CHEMBL5271186 1 7.55
CHEMBL5289131 1 7.54
CHEMBL5277043 1 7.48
CHEMBL5280825 1 7.25
CHEMBL5286238 1 7.17
CHEMBL5272422 1 7.16
CHEMBL5273713 1 7.00
CHEMBL5280350 1 6.72
CHEMBL5279010 1 6.67
CHEMBL5270999 1 6.57
CHEMBL5278425 1 6.45
CHEMBL5281152 1 6.37
CHEMBL5279534 1 6.30
CHEMBL5272665 1 6.27
CHEMBL5274593 1 6.20
CHEMBL5286408 1 6.16
CHEMBL5289173 1 6.11
CHEMBL5274485 1 6.11
CHEMBL5287497 1 5.98
CHEMBL5277535 1 5.85
CHEMBL5276852 1 5.83
CHEMBL5274190 1 -
CHEMBL5286388 1 -
CHEMBL5288208 1 -
CHEMBL5288741 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5289179 IC50 = 0.6 nM 9.22
CHEMBL5286478 IC50 = 4.0 nM 8.40
CHEMBL5272300 IC50 = 15.0 nM 7.82
CHEMBL5278493 IC50 = 16.0 nM 7.80
CHEMBL5277818 IC50 = 22.0 nM 7.66
CHEMBL5271186 IC50 = 28.0 nM 7.55
CHEMBL5289131 IC50 = 29.0 nM 7.54
CHEMBL5277043 IC50 = 33.0 nM 7.48
CHEMBL5280825 IC50 = 56.0 nM 7.25
CHEMBL5286238 IC50 = 67.0 nM 7.17
CHEMBL5272422 IC50 = 70.0 nM 7.16
CHEMBL5273713 IC50 = 99.0 nM 7.00
CHEMBL5280350 IC50 = 190.0 nM 6.72
CHEMBL5279010 IC50 = 216.0 nM 6.67
CHEMBL5270999 IC50 = 268.0 nM 6.57
CHEMBL5278425 IC50 = 357.0 nM 6.45
CHEMBL5281152 IC50 = 430.0 nM 6.37
CHEMBL5279534 IC50 = 498.0 nM 6.30
CHEMBL5272665 IC50 = 535.0 nM 6.27
CHEMBL5274593 IC50 = 636.0 nM 6.20
CHEMBL5286408 IC50 = 695.0 nM 6.16
CHEMBL5289173 IC50 = 774.0 nM 6.11
CHEMBL5274485 IC50 = 779.0 nM 6.11
CHEMBL5287497 IC50 = 1053.0 nM 5.98
CHEMBL5277535 IC50 = 1415.0 nM 5.85
CHEMBL5276852 IC50 = 1474.0 nM 5.83
CHEMBL5274190 IC50 > 10000.0 nM -
CHEMBL5286388 IC50 > 10000.0 nM -
CHEMBL5288208 IC50 > 10000.0 nM -
CHEMBL5288741 IC50 > 10000.0 nM -