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Assay Detail

CHEMBL5251999

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]DTG from sigma 2 receptor in Wistar Han rat liver membrane assessed as inhibition constant incubated for 120 mins
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sigma intracellular receptor 2 (CHEMBL4105864)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Development of Fluorescent 4-[4-(3<i>H</i>-Spiro[isobenzofuran-1,4'-piperidin]-1'-yl)butyl]indolyl Derivatives as High-Affinity Probes to Enable the Study of σ Receptors via Fluorescence-Based Techniques.
Abatematteo FS, Majellaro M, Montsch B, Prieto-Díaz R, Niso M, Contino M, Stefanachi A, Riganti C, Mangiatordi GF, Delre P, Heffeter P, Sotelo E, Abate C.
J Med Chem (2023) 66 : 3798 -3817
PubMed 36919956 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 7.82 9.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5271751 1 9.37
CHEMBL5274668 1 8.42
CHEMBL5284531 1 8.29
CHEMBL5280109 1 8.00
CHEMBL61479 SIRAMESINE 2.0 1 7.90
CHEMBL282433 DITOLYLGUANIDINE 1 7.71
CHEMBL5290452 1 7.52
CHEMBL5286943 1 7.41
CHEMBL5281223 1 7.40
CHEMBL5278003 1 7.29
CHEMBL5278417 1 6.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5271751 Ki = 0.43 nM 9.37
CHEMBL5274668 Ki = 3.84 nM 8.42
CHEMBL5284531 Ki = 5.07 nM 8.29
CHEMBL5280109 Ki = 10.1 nM 8.00
CHEMBL61479 SIRAMESINE Ki = 12.6 nM 7.90
CHEMBL282433 DITOLYLGUANIDINE Ki = 19.5 nM 7.71
CHEMBL5290452 Ki = 30.2 nM 7.52
CHEMBL5286943 Ki = 39.4 nM 7.41
CHEMBL5281223 Ki = 39.8 nM 7.40
CHEMBL5278003 Ki = 51.1 nM 7.29
CHEMBL5278417 Ki = 220.0 nM 6.66