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Assay Detail

CHEMBL5256649

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST tagged recombinant human VEGFR2 (aa805 to 1356 residues) expressed in baculovirus expression system using Poly- (Glu4:Tyr)-biotin as substrate incubated for 80 mins in presence of ATP by kinase glo method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vascular endothelial growth factor receptor 2 (CHEMBL279)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Futibatinib: The First Covalent FGFR Kinase Inhibitor in Clinical Use.
Ito S, Otsuki S, Ohsawa H, Hirano A, Kazuno H, Yamashita S, Egami K, Shibata Y, Yamamiya I, Yamashita F, Kodama Y, Funabashi K, Kazuno H, Komori T, Suzuki S, Sootome H, Hirai H, Sagara T.
ACS Med Chem Lett (2023) 14 : 396 -404
PubMed 37077386 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.59 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5278505 1 5.92
CHEMBL5287545 1 5.83
CHEMBL3701255 1 5.75
CHEMBL3701238 FUTIBATINIB 4.0 1 5.63
CHEMBL3701241 1 5.52
CHEMBL5269016 1 5.47
CHEMBL3701243 1 5.43
CHEMBL5268467 1 5.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5278505 IC50 = 1206.0 nM 5.92
CHEMBL5287545 IC50 = 1486.0 nM 5.83
CHEMBL3701255 IC50 = 1770.0 nM 5.75
CHEMBL3701238 FUTIBATINIB IC50 = 2358.0 nM 5.63
CHEMBL3701241 IC50 = 3029.0 nM 5.52
CHEMBL5269016 IC50 = 3377.0 nM 5.47
CHEMBL3701243 IC50 = 3686.0 nM 5.43
CHEMBL5268467 IC50 = 7010.0 nM 5.15