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Assay Detail

CHEMBL5259462

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human GSK3beta (636 to 661 residues) assessed as reduction in human muscle glycogen synthase type 1 fragment phosphorylation using YRRAAVPPSPSLSRHSSPHQ(pS)EDE as substrate in presence of ATP incubated for 60 mins by ADP-Glo kinase assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

3-Arylidene-2-oxindoles as GSK3β inhibitors and anti-thrombotic agents.
Babkov D, Bezsonova E, Sirotenko V, Othman E, Klochkov V, Sosonyuk S, Lozinskaya N, Spasov A.
Bioorg Med Chem Lett (2023) 87 : 129283 -129283
PubMed 37054760 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.80 8.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5284455 1 8.38
CHEMBL102714 SB-216763 1 8.08
CHEMBL5284219 1 6.81
CHEMBL5276187 1 6.63
CHEMBL5272429 1 6.46
CHEMBL4440139 1 5.91
CHEMBL5202388 1 5.76
CHEMBL5274127 1 5.75
CHEMBL5266960 1 5.36
CHEMBL5289425 1 5.25
CHEMBL5270462 1 5.17
CHEMBL2347086 1 4.89
CHEMBL5274095 1 4.87
CHEMBL5266917 1 4.70
CHEMBL5285483 1 4.46
CHEMBL5185154 1 4.30
CHEMBL5271237 1 -
CHEMBL5280912 1 -
CHEMBL5271795 1 -
CHEMBL103307 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5284455 IC50 = 4.2 nM 8.38
CHEMBL102714 SB-216763 IC50 = 8.4 nM 8.08
CHEMBL5284219 IC50 = 155.0 nM 6.81
CHEMBL5276187 IC50 = 233.0 nM 6.63
CHEMBL5272429 IC50 = 348.0 nM 6.46
CHEMBL4440139 IC50 = 1231.0 nM 5.91
CHEMBL5202388 IC50 = 1717.0 nM 5.76
CHEMBL5274127 IC50 = 1770.0 nM 5.75
CHEMBL5266960 IC50 = 4343.0 nM 5.36
CHEMBL5289425 IC50 = 5680.0 nM 5.25
CHEMBL5270462 IC50 = 6720.0 nM 5.17
CHEMBL2347086 IC50 = 12750.0 nM 4.89
CHEMBL5274095 IC50 = 13490.0 nM 4.87
CHEMBL5266917 IC50 = 20010.0 nM 4.70
CHEMBL5285483 IC50 = 34440.0 nM 4.46
CHEMBL5185154 IC50 = 50000.0 nM 4.30
CHEMBL5271237 IC50 > 50000.0 nM -
CHEMBL5280912 IC50 > 50000.0 nM -
CHEMBL5271795 IC50 > 50000.0 nM -
CHEMBL103307 IC50 > 50000.0 nM -