Assay Detail
Binding
CHEMBL5259642
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Competitive binding affinity to Nluc-fused human GPR35 expressed in CHO-K1 cells assessed as inhibition constant using furimazine as substrate incubated for 30 mins followed by substrate addition by in presence of 10-(4-(2-(4-(4-((2,8-dicarboxy-6-hydroxy-10-methylpyrido[3,2-g]quinolin-4-yl)oxy)butyl)-1H-1,2,3-triazol-1-yl)ethoxy)phenyl)-5,5-difluoro-2,7,9-trimethyl-5H-imidazo[1,2-c]pyrrolo[2,1-f][1,3,2]diazaborinin-6-ium-5-uide
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | CHO-K1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Development and Characterization of Fluorescent Probes for the G Protein-Coupled Receptor 35.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 11 | 7.13 | 8.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1384502 | — | — | 1 | 8.40 |
| CHEMBL3306990 | — | — | 1 | 8.22 |
| CHEMBL4175848 | — | — | 1 | 8.00 |
| CHEMBL177880 | PAMOIC ACID | — | 1 | 7.64 |
| CHEMBL4878979 | — | — | 1 | 7.55 |
| CHEMBL4100677 | — | — | 1 | 7.29 |
| CHEMBL1708510 | — | — | 1 | 6.63 |
| CHEMBL1235421 | PYRROLOQUINOLINE QUINONE | — | 1 | 6.48 |
| CHEMBL4081238 | — | — | 1 | 6.45 |
| CHEMBL28079 | ZAPRINAST | 2.0 | 1 | 6.21 |
| CHEMBL428880 | CROMOLYN | 4.0 | 1 | 5.53 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1384502 | — | Ki | = | 4.0 | nM | 8.40 |
| CHEMBL3306990 | — | Ki | = | 6.0 | nM | 8.22 |
| CHEMBL4175848 | — | Ki | = | 10.0 | nM | 8.00 |
| CHEMBL177880 | PAMOIC ACID | Ki | = | 23.0 | nM | 7.64 |
| CHEMBL4878979 | — | Ki | = | 28.0 | nM | 7.55 |
| CHEMBL4100677 | — | Ki | = | 51.0 | nM | 7.29 |
| CHEMBL1708510 | — | Ki | = | 236.0 | nM | 6.63 |
| CHEMBL1235421 | PYRROLOQUINOLINE QUINONE | Ki | = | 332.0 | nM | 6.48 |
| CHEMBL4081238 | — | Ki | = | 352.0 | nM | 6.45 |
| CHEMBL28079 | ZAPRINAST | Ki | = | 612.0 | nM | 6.21 |
| CHEMBL428880 | CROMOLYN | Ki | = | 2970.0 | nM | 5.53 |