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Assay Detail

CHEMBL5259648

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive binding affinity to Nluc-fused human GPR35 expressed in CHO-K1 cells assessed as inhibition constant using furimazine as substrate incubated for 30 mins followed by substrate addition by in presence of 50 nM of 10-(4-(2-(4-(4-((2,8-dicarboxy-6-hydroxy-10-methylpyrido[3,2-g]quinolin-4-yl)oxy)butyl)-1H-1,2,3-triazol-1-yl)ethoxy)phenyl)-5,5-difluoro-2,7,9-trimethyl-5H-imidazo[1,2-c]pyrrolo[2,1-f][1,3,2]diazaborinin-6-ium-5-uide
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 35 (CHEMBL1293267)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and Characterization of Fluorescent Probes for the G Protein-Coupled Receptor 35.
Wei L, Xiang K, Kang H, Yu Y, Fan H, Zhou H, Hou T, Ge Y, Wang J, Guo Z, Chen Y, Zhao Y, Liang X.
ACS Med Chem Lett (2023) 14 : 411 -416
PubMed 37077394 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 7.99 7.99

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL177880 PAMOIC ACID 1 7.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL177880 PAMOIC ACID Ki = 10.3 nM 7.99