Assay Detail
Binding
CHEMBL5259686
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of wild type EGFR (unknown origin) pre-incubated for 10 mins followed by substrate addition measured after 60 mins in presence of ATP by mobility shifting assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and evaluation of sulfonamide derivatives targeting EGFR<sup>790M/L858R</sup> mutations and ALK rearrangement as anticancer agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 8.52 | 10.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1173655 | AFATINIB | 4.0 | 1 | 10.10 |
| CHEMBL3353410 | OSIMERTINIB | 4.0 | 1 | 7.83 |
| CHEMBL5284780 | — | — | 1 | 7.63 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1173655 | AFATINIB | IC50 | = | 0.08 | nM | 10.10 |
| CHEMBL3353410 | OSIMERTINIB | IC50 | = | 14.67 | nM | 7.83 |
| CHEMBL5284780 | — | IC50 | = | 23.25 | nM | 7.63 |