Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5260456

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R mutant (unknown origin) using TK-substrate preincubated with enzyme for 30 mins followed by substrate and ATP addition for 15 mins by HTRF assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Addressing the Osimertinib Resistance Mutation EGFR-L858R/C797S with Reversible Aminopyrimidines.
Grabe T, Jeyakumar K, Niggenaber J, Schulz T, Koska S, Kleinbölting S, Beck ME, Müller MP, Rauh D.
ACS Med Chem Lett (2023) 14 : 591 -598
PubMed 37197473 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 9.50 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4650319 MOBOCERTINIB 4.0 1 11.00
CHEMBL3353410 OSIMERTINIB 4.0 1 10.30
CHEMBL5275642 1 9.77
CHEMBL5278657 1 9.17
CHEMBL5269375 1 8.47
CHEMBL5285308 1 8.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4650319 MOBOCERTINIB IC50 = 0.01 nM 11.00
CHEMBL3353410 OSIMERTINIB IC50 = 0.05 nM 10.30
CHEMBL5275642 IC50 = 0.17 nM 9.77
CHEMBL5278657 IC50 = 0.68 nM 9.17
CHEMBL5269375 IC50 = 3.4 nM 8.47
CHEMBL5285308 IC50 = 5.4 nM 8.27