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Assay Detail

CHEMBL5260458

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R/T790M/C797S mutant (unknown origin) using TK-substrate preincubated with enzyme for 30 mins followed by substrate and ATP addition for 10 mins by HTRF assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Addressing the Osimertinib Resistance Mutation EGFR-L858R/C797S with Reversible Aminopyrimidines.
Grabe T, Jeyakumar K, Niggenaber J, Schulz T, Koska S, Kleinbölting S, Beck ME, Müller MP, Rauh D.
ACS Med Chem Lett (2023) 14 : 591 -598
PubMed 37197473 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.68 9.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5270693 1 9.19
CHEMBL3545311 BRIGATINIB 4.0 1 8.89
CHEMBL5285052 1 8.21
CHEMBL5284924 1 8.06
CHEMBL5287229 1 7.92
CHEMBL5289859 1 7.82
CHEMBL5280082 1 7.72
CHEMBL5275642 1 7.46
CHEMBL5288276 1 7.11
CHEMBL3353410 OSIMERTINIB 4.0 1 6.82
CHEMBL553 ERLOTINIB 4.0 1 6.50
CHEMBL4650319 MOBOCERTINIB 4.0 1 6.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5270693 IC50 = 0.64 nM 9.19
CHEMBL3545311 BRIGATINIB IC50 = 1.3 nM 8.89
CHEMBL5285052 IC50 = 6.2 nM 8.21
CHEMBL5284924 IC50 = 8.8 nM 8.06
CHEMBL5287229 IC50 = 12.0 nM 7.92
CHEMBL5289859 IC50 = 15.0 nM 7.82
CHEMBL5280082 IC50 = 19.0 nM 7.72
CHEMBL5275642 IC50 = 35.0 nM 7.46
CHEMBL5288276 IC50 = 78.0 nM 7.11
CHEMBL3353410 OSIMERTINIB IC50 = 151.0 nM 6.82
CHEMBL553 ERLOTINIB IC50 = 313.0 nM 6.50
CHEMBL4650319 MOBOCERTINIB IC50 = 364.0 nM 6.44