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Assay Detail

CHEMBL5260461

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human A-431 cells expressing wild type EGFR assessed as reduction in cell viability incubated for 96 hrs by CellTiter-Glo assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type A-431
Confidence 1 — Organism
Curated By Autocuration

Target

A-431 (CHEMBL614069)
Type CELL-LINE
Organism Homo sapiens

Publication

Addressing the Osimertinib Resistance Mutation EGFR-L858R/C797S with Reversible Aminopyrimidines.
Grabe T, Jeyakumar K, Niggenaber J, Schulz T, Koska S, Kleinbölting S, Beck ME, Müller MP, Rauh D.
ACS Med Chem Lett (2023) 14 : 591 -598
PubMed 37197473 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 5.61 6.08

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3353410 OSIMERTINIB 4.0 1 6.08
CHEMBL3545311 BRIGATINIB 4.0 1 6.03
CHEMBL5285052 1 5.85
CHEMBL4650319 MOBOCERTINIB 4.0 1 5.78
CHEMBL5270693 1 5.74
CHEMBL5284924 1 5.58
CHEMBL553 ERLOTINIB 4.0 1 5.55
CHEMBL5280082 1 5.43
CHEMBL5288276 1 5.41
CHEMBL5289859 1 5.41
CHEMBL5287229 1 5.40
CHEMBL5275642 1 5.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3353410 OSIMERTINIB EC50 = 830.0 nM 6.08
CHEMBL3545311 BRIGATINIB EC50 = 936.0 nM 6.03
CHEMBL5285052 EC50 = 1414.0 nM 5.85
CHEMBL4650319 MOBOCERTINIB EC50 = 1676.0 nM 5.78
CHEMBL5270693 EC50 = 1837.0 nM 5.74
CHEMBL5284924 EC50 = 2607.0 nM 5.58
CHEMBL553 ERLOTINIB EC50 = 2805.0 nM 5.55
CHEMBL5280082 EC50 = 3669.0 nM 5.43
CHEMBL5288276 EC50 = 3915.0 nM 5.41
CHEMBL5289859 EC50 = 3887.0 nM 5.41
CHEMBL5287229 EC50 = 4016.0 nM 5.40
CHEMBL5275642 EC50 = 7956.0 nM 5.10