Assay Detail
Binding
CHEMBL5318939
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human full length-His6 tagged PIM1 (1 to 312 residues) expressed in Escherichia coli BL21 (DE3) assessed as inhibition constant using RSRHSSYPAGT as peptide substrate incubated for 10 mins in presence of [gamma-32P]ATP by radiometric scintillation assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase pim-1 (CHEMBL2147) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Targeting Death-Associated Protein Kinases for Treatment of Human Diseases: Recent Advances and Future Directions.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 4 | 5.44 | 5.83 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5425555 | — | — | 1 | 5.83 |
| CHEMBL5303531 | — | — | 1 | 5.53 |
| CHEMBL5413882 | — | — | 1 | 5.20 |
| CHEMBL5410656 | — | — | 1 | 5.19 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5425555 | — | Ki | = | 1480.0 | nM | 5.83 |
| CHEMBL5303531 | — | Ki | = | 2940.0 | nM | 5.53 |
| CHEMBL5413882 | — | Ki | = | 6310.0 | nM | 5.20 |
| CHEMBL5410656 | — | Ki | = | 6480.0 | nM | 5.19 |