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Assay Detail

CHEMBL5319830

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Negative allosteric modulation of human GluN1A/GluN2B receptor expressed in T-REX-CHO cells preincubated for 5 mins followed by glutamate and glycine addition and measured after 5 mins by FLIPR assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type T-REX-CHO
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2B (CHEMBL1907603)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of a Series of Substituted 1<i>H</i>-((1,2,3-Triazol-4-yl)methoxy)pyrimidines as Brain Penetrants and Potent GluN2B-Selective Negative Allosteric Modulators.
Gelin CF, Stenne B, Coate H, Hiscox A, Soyode-Johnson A, Wall JL, Lord B, Schoellerman J, Coe KJ, Wang K, Alcázar J, Chrovian CC, Dvorak CA, Carruthers NI, Koudriakova T, Balana B, Letavic MA.
J Med Chem (2023) 66 : 2877 -2892
PubMed 36757100 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.17 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5410727 1 8.72
CHEMBL5397778 1 8.46
CHEMBL5433088 1 8.11
CHEMBL5396805 1 8.08
CHEMBL5438508 1 7.96
CHEMBL5433511 1 7.96
CHEMBL5421096 1 7.96
CHEMBL5409770 1 7.80
CHEMBL5413455 1 7.80
CHEMBL5438363 1 7.80
CHEMBL5414178 1 7.60
CHEMBL5425798 1 7.31
CHEMBL5432620 1 7.23
CHEMBL5401297 1 6.89
CHEMBL5399984 1 6.44
CHEMBL5419741 1 6.41
CHEMBL5426084 1 6.30
CHEMBL5404498 1 6.24
CHEMBL5432367 1 6.22
CHEMBL5436548 1 6.11
CHEMBL5395318 1 6.08
CHEMBL5397475 1 5.92
CHEMBL5433455 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5410727 IC50 = 1.9 nM 8.72
CHEMBL5397778 IC50 = 3.5 nM 8.46
CHEMBL5433088 IC50 = 7.8 nM 8.11
CHEMBL5396805 IC50 = 8.3 nM 8.08
CHEMBL5438508 IC50 = 11.0 nM 7.96
CHEMBL5433511 IC50 = 11.0 nM 7.96
CHEMBL5421096 IC50 = 11.0 nM 7.96
CHEMBL5409770 IC50 = 16.0 nM 7.80
CHEMBL5413455 IC50 = 16.0 nM 7.80
CHEMBL5438363 IC50 = 16.0 nM 7.80
CHEMBL5414178 IC50 = 25.0 nM 7.60
CHEMBL5425798 IC50 = 49.0 nM 7.31
CHEMBL5432620 IC50 = 59.0 nM 7.23
CHEMBL5401297 IC50 = 130.0 nM 6.89
CHEMBL5399984 IC50 = 360.0 nM 6.44
CHEMBL5419741 IC50 = 390.0 nM 6.41
CHEMBL5426084 IC50 = 500.0 nM 6.30
CHEMBL5404498 IC50 = 580.0 nM 6.24
CHEMBL5432367 IC50 = 600.0 nM 6.22
CHEMBL5436548 IC50 = 770.0 nM 6.11
CHEMBL5395318 IC50 = 840.0 nM 6.08
CHEMBL5397475 IC50 = 1200.0 nM 5.92
CHEMBL5433455 IC50 = 2500.0 nM 5.60