Assay Detail
Binding
CHEMBL5320451
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Nano-Luc fused PIKFYVE (unknown origin) expressed in HEK293T cells pretreated for 3 hrs followed by K8-tarcer addition and measured after 1 hr by NanoBRET assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
1-phosphatidylinositol 3-phosphate 5-kinase (CHEMBL1938222) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Development of a Covalent Inhibitor of c-Jun N-Terminal Protein Kinase (JNK) 2/3 with Selectivity over JNK1.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.47 | 8.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4297643 | APILIMOD | 2.0 | 1 | 8.40 |
| CHEMBL5413843 | — | — | 1 | 6.54 |
| CHEMBL5398852 | — | — | 1 | - |
| CHEMBL5415114 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4297643 | APILIMOD | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5413843 | — | IC50 | = | 292.0 | nM | 6.54 |
| CHEMBL5398852 | — | IC50 | > | 3000.0 | nM | - |
| CHEMBL5415114 | — | IC50 | > | 3000.0 | nM | - |