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Assay Detail

CHEMBL5320992

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS CoV-2 main protease preincubated for 30 min followed by substrate addition and measured after 60 mins by fluorescence assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Discovery of novel bicyclic[3.3.0]proline peptidyl α-ketoamides as potent 3CL-protease inhibitors for SARS-CoV-2.
Chen X, Li P, Huang J, Yang Y, Zhang H, Wang Z, Zhu Z, Wang J, Zhang J, Chen K, He H, Long C, Chen S.
Bioorg Med Chem Lett (2023) 90 : 129324 -129324
PubMed 37182612 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.07 7.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4802135 NIRMATRELVIR 4.0 1 7.85
CHEMBL5403964 1 7.47
CHEMBL5419985 1 7.37
CHEMBL5439916 1 7.33
CHEMBL5409804 1 7.30
CHEMBL5414443 1 7.11
CHEMBL5438575 1 6.98
CHEMBL5400701 1 6.87
CHEMBL5402227 1 6.78
CHEMBL5403340 1 6.45
CHEMBL5411271 1 6.26
CHEMBL5400782 1 -
CHEMBL5430131 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4802135 NIRMATRELVIR IC50 = 14.0 nM 7.85
CHEMBL5403964 IC50 = 34.0 nM 7.47
CHEMBL5419985 IC50 = 43.0 nM 7.37
CHEMBL5439916 IC50 = 47.0 nM 7.33
CHEMBL5409804 IC50 = 50.0 nM 7.30
CHEMBL5414443 IC50 = 77.0 nM 7.11
CHEMBL5438575 IC50 = 104.0 nM 6.98
CHEMBL5400701 IC50 = 135.0 nM 6.87
CHEMBL5402227 IC50 = 165.0 nM 6.78
CHEMBL5403340 IC50 = 356.0 nM 6.45
CHEMBL5411271 IC50 = 551.0 nM 6.26
CHEMBL5400782 IC50 > 1000.0 nM -
CHEMBL5430131 IC50 > 1000.0 nM -