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Assay Detail

CHEMBL5324504

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-1,3-di-O-tolylguanidine from human sigma 2 receptor/TMEM97 transfected in sigma 1 receptor knockout HEK293 cell membranes assessed as inhibition constant measured after 120 mins by microbeta scintillation counting analysis
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sigma intracellular receptor 2 (CHEMBL4105907)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of WLB-89462, a New Drug-like and Highly Selective σ<sub>2</sub> Receptor Ligand with Neuroprotective Properties.
Christmann U, Díaz JL, Pascual R, Bordas M, Álvarez I, Monroy X, Porras M, Yeste S, Reinoso RF, Merlos M, Vela JM, Almansa C.
J Med Chem (2023) 66 : 12499 -12519
PubMed 37607512 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 26 7.35 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5418939 1 9.00
CHEMBL5395523 1 9.00
CHEMBL5393813 1 8.52
CHEMBL5425053 1 8.40
CHEMBL4846092 1 8.30
CHEMBL5418322 1 8.30
CHEMBL5427726 1 8.22
CHEMBL5401020 1 7.89
CHEMBL5424034 1 7.42
CHEMBL5394618 1 7.33
CHEMBL5398618 1 7.23
CHEMBL5400448 1 7.21
CHEMBL5426449 1 7.21
CHEMBL5426097 1 7.01
CHEMBL5433551 1 6.94
CHEMBL5438422 1 6.88
CHEMBL5395314 1 6.79
CHEMBL5395635 1 6.50
CHEMBL5419738 1 6.34
CHEMBL5416073 1 6.30
CHEMBL5430674 1 6.14
CHEMBL5435310 1 6.10
CHEMBL5411022 1 6.02
CHEMBL5400873 1 -
CHEMBL5394360 1 -
CHEMBL5432378 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5418939 Ki = 1.0 nM 9.00
CHEMBL5395523 Ki = 1.0 nM 9.00
CHEMBL5393813 Ki = 3.0 nM 8.52
CHEMBL5425053 Ki = 4.0 nM 8.40
CHEMBL4846092 Ki = 5.0 nM 8.30
CHEMBL5418322 Ki = 5.0 nM 8.30
CHEMBL5427726 Ki = 6.0 nM 8.22
CHEMBL5401020 Ki = 13.0 nM 7.89
CHEMBL5424034 Ki = 38.0 nM 7.42
CHEMBL5394618 Ki = 47.0 nM 7.33
CHEMBL5398618 Ki = 59.0 nM 7.23
CHEMBL5400448 Ki = 61.0 nM 7.21
CHEMBL5426449 Ki = 62.0 nM 7.21
CHEMBL5426097 Ki = 98.0 nM 7.01
CHEMBL5433551 Ki = 116.0 nM 6.94
CHEMBL5438422 Ki = 131.0 nM 6.88
CHEMBL5395314 Ki = 164.0 nM 6.79
CHEMBL5395635 Ki = 319.0 nM 6.50
CHEMBL5419738 Ki = 457.0 nM 6.34
CHEMBL5416073 Ki = 496.0 nM 6.30
CHEMBL5430674 Ki = 727.0 nM 6.14
CHEMBL5435310 Ki = 788.0 nM 6.10
CHEMBL5411022 Ki = 951.0 nM 6.02
CHEMBL5400873 Ki > 1000.0 nM -
CHEMBL5394360 Ki > 1000.0 nM -
CHEMBL5432378 Ki > 1000.0 nM -