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Assay Detail

CHEMBL5326439

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity against ERalpha in estradiol stimulated HEK293T cells measuring luciferase activity after 24 hrs by luciferase reporter assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-guided identification of novel dual-targeting estrogen receptor α degraders with aromatase inhibitory activity for the treatment of endocrine-resistant breast cancer.
Xin L, Min J, Hu H, Li Y, Du C, Xie B, Cheng Y, Deng X, Deng X, Shen K, Huang J, Chen CC, Guo RT, Dong C, Zhou HB.
Eur J Med Chem (2023) 253 : 115328 -115328
PubMed 37037140 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 8.11 10.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL489 AFIMOXIFENE 3.0 1 10.01
CHEMBL1358 FULVESTRANT 4.0 1 9.38
CHEMBL5404063 1 7.40
CHEMBL5412790 1 7.27
CHEMBL5405944 1 6.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL489 AFIMOXIFENE EC50 = 0.0987 nM 10.01
CHEMBL1358 FULVESTRANT EC50 = 0.418 nM 9.38
CHEMBL5404063 EC50 = 39.6 nM 7.40
CHEMBL5412790 EC50 = 53.5 nM 7.27
CHEMBL5405944 EC50 = 332.0 nM 6.48