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Assay Detail

CHEMBL5332565

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His-tagged BLM (unknown origin) expressed in Escherichia coli BL21 (DE3) using 5'-biotin-labeled duplex forked-DNA as substrate incubated for 10 mins followed by substrate addition measured after 30 mins in presence of ATP by electrophoretic mobility shift assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RecQ-like DNA helicase BLM (CHEMBL1293237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and evaluation of N3-substituted quinazolinone derivatives as potential Bloom's Syndrome protein (BLM) helicase inhibitor for sensitization treatment of colorectal cancer.
Tu JL, Wu BH, Wu HB, Wang JE, Zhang ZL, Gao KY, Zhang LX, Chen QR, Zhou YC, Tan JH, Huang ZS, Chen SB.
Eur J Med Chem (2023) 246 : 114944 -114944
PubMed 36459756 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 5.33 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5423610 1 6.10
CHEMBL5440285 1 5.89
CHEMBL5424781 1 5.64
CHEMBL5433468 1 5.60
CHEMBL5437731 1 5.54
CHEMBL5433068 1 5.43
CHEMBL5425282 1 5.40
CHEMBL5405727 1 5.37
CHEMBL5417970 1 5.32
CHEMBL5411202 1 5.30
CHEMBL4633662 1 5.29
CHEMBL5436428 1 5.28
CHEMBL5408314 1 5.28
CHEMBL5408479 1 5.28
CHEMBL5439207 1 5.24
CHEMBL5394652 1 5.10
CHEMBL5410036 1 5.08
CHEMBL5439537 1 5.04
CHEMBL5397042 1 5.01
CHEMBL5430184 1 4.50
CHEMBL5419427 1 -
CHEMBL5408083 1 -
CHEMBL5406659 1 -
CHEMBL5409326 1 -
CHEMBL5436259 1 -
CHEMBL5398979 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5423610 IC50 = 800.0 nM 6.10
CHEMBL5440285 IC50 = 1300.0 nM 5.89
CHEMBL5424781 IC50 = 2300.0 nM 5.64
CHEMBL5433468 IC50 = 2500.0 nM 5.60
CHEMBL5437731 IC50 = 2900.0 nM 5.54
CHEMBL5433068 IC50 = 3700.0 nM 5.43
CHEMBL5425282 IC50 = 4000.0 nM 5.40
CHEMBL5405727 IC50 = 4300.0 nM 5.37
CHEMBL5417970 IC50 = 4800.0 nM 5.32
CHEMBL5411202 IC50 = 5000.0 nM 5.30
CHEMBL4633662 IC50 = 5100.0 nM 5.29
CHEMBL5436428 IC50 = 5300.0 nM 5.28
CHEMBL5408314 IC50 = 5200.0 nM 5.28
CHEMBL5408479 IC50 = 5200.0 nM 5.28
CHEMBL5439207 IC50 = 5800.0 nM 5.24
CHEMBL5394652 IC50 = 8000.0 nM 5.10
CHEMBL5410036 IC50 = 8300.0 nM 5.08
CHEMBL5439537 IC50 = 9100.0 nM 5.04
CHEMBL5397042 IC50 = 9700.0 nM 5.01
CHEMBL5430184 IC50 = 32000.0 nM 4.50
CHEMBL5419427 IC50 > 32000.0 nM -
CHEMBL5408083 IC50 > 32000.0 nM -
CHEMBL5406659 IC50 > 32000.0 nM -
CHEMBL5409326 IC50 > 32000.0 nM -
CHEMBL5436259 IC50 > 32000.0 nM -
CHEMBL5398979 IC50 > 32000.0 nM -