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Assay Detail

CHEMBL5333103

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV-2 MPro using Dabcyl-KTSAVLQSGFRKME-Edans as substrate assessed as inhibition constant measured for 10 mins by FRET assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Structure-based lead optimization of peptide-based vinyl methyl ketones as SARS-CoV-2 main protease inhibitors.
Previti S, Ettari R, Calcaterra E, Di Maro S, Hammerschmidt SJ, Müller C, Ziebuhr J, Schirmeister T, Cosconati S, Zappalà M.
Eur J Med Chem (2023) 247 : 115021 -115021
PubMed 36549112 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.89 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4802135 NIRMATRELVIR 4.0 1 8.52
CHEMBL4796938 1 8.10
CHEMBL5436027 1 6.74
CHEMBL5423619 1 6.60
CHEMBL5401213 1 6.58
CHEMBL5412724 1 6.41
CHEMBL5429672 1 6.38
CHEMBL5402020 1 5.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4802135 NIRMATRELVIR Ki = 3.0 nM 8.52
CHEMBL4796938 Ki = 8.0 nM 8.10
CHEMBL5436027 Ki = 184.0 nM 6.74
CHEMBL5423619 Ki = 252.0 nM 6.60
CHEMBL5401213 Ki = 260.0 nM 6.58
CHEMBL5412724 Ki = 386.0 nM 6.41
CHEMBL5429672 Ki = 416.0 nM 6.38
CHEMBL5402020 Ki = 1770.0 nM 5.75