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Assay Detail

CHEMBL5334563

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human placental microsomal fraction 17beta-HSD2 using [3H]E2 as substrate measured after 20 mins in presence of NAD+ by HPLC analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Placenta
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

17-beta-hydroxysteroid dehydrogenase type 2 (CHEMBL2789)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent Dual Inhibitors of Steroid Sulfatase and 17β-Hydroxysteroid Dehydrogenase Type 1 with a Suitable Pharmacokinetic Profile for <i>In Vivo</i> Proof-of-Principle Studies in an Endometriosis Mouse Model.
Salah M, Tahoun M, Rudzitis-Auth J, Stotz L, van Koppen CJ, Laschke MW, Abdelsamie AS, Frotscher M.
J Med Chem (2023) 66 : 8975 -8992
PubMed 37369108 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.56 7.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4071245 1 7.44
CHEMBL5431739 1 5.62
CHEMBL5080299 1 5.50
CHEMBL5440694 1 5.50
CHEMBL5440157 1 5.36
CHEMBL5429832 1 5.21
CHEMBL5417947 1 5.19
CHEMBL5394384 1 5.12
CHEMBL5394095 1 5.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4071245 IC50 = 36.0 nM 7.44
CHEMBL5431739 IC50 = 2400.0 nM 5.62
CHEMBL5080299 IC50 = 3200.0 nM 5.50
CHEMBL5440694 IC50 = 3200.0 nM 5.50
CHEMBL5440157 IC50 = 4400.0 nM 5.36
CHEMBL5429832 IC50 = 6200.0 nM 5.21
CHEMBL5417947 IC50 = 6400.0 nM 5.19
CHEMBL5394384 IC50 = 7500.0 nM 5.12
CHEMBL5394095 IC50 = 7700.0 nM 5.11