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Assay Detail

CHEMBL5336136

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Cathepsin L using HiLyte Fluor488TM as substrate pre-incubated for 60 mins in presence of GSH followed by substrate addition measured after 30 mins by fluorescence based assay
6
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Procathepsin L (CHEMBL3837)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel dithiocarbamates selectively inhibit 3CL protease of SARS-CoV-2 and other coronaviruses.
Brier L, Hassan H, Hanoulle X, Landry V, Moschidi D, Desmarets L, Rouillé Y, Dumont J, Herledan A, Warenghem S, Piveteau C, Carré P, Ikherbane S, Cantrelle FX, Dupré E, Dubuisson J, Belouzard S, Leroux F, Deprez B, Charton J.
Eur J Med Chem (2023) 250 : 115186 -115186
PubMed 36796300 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.73 9.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2365410 2 9.16
CHEMBL5436771 2 4.37
CHEMBL4590273 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2365410 IC50 = 0.6918 nM 9.16
CHEMBL2365410 IC50 = 1.0 nM 9.00
CHEMBL5436771 IC50 = 42800.0 nM 4.37
CHEMBL5436771 IC50 = 42657.95 nM 4.37
CHEMBL4590273 IC50 = 122000.0 nM -
CHEMBL4590273 IC50 = 123026.88 nM -