Assay Detail
Binding
CHEMBL5336367
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of His-tagged SARS-CoV-2 Main protease BetaCoV/Wuhan/WIV04/2019 expressed in Escherichia coli BL21 (DE3) using Dabcyl-KTSAVLQ/SGFRKME(Edan) as substrate followed by compound addition by FRET assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1ab (CHEMBL4523582) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
Publication
The research progress of SARS-CoV-2 main protease inhibitors from 2020 to 2022.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.00 | 7.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3427166 | — | — | 1 | 7.52 |
| CHEMBL4868443 | — | — | 1 | 7.16 |
| CHEMBL4860690 | — | — | 1 | 7.10 |
| CHEMBL4850660 | — | — | 1 | 6.75 |
| CHEMBL5430984 | — | — | 1 | 6.75 |
| CHEMBL4856219 | — | — | 1 | 6.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3427166 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL4868443 | — | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL4860690 | — | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL4850660 | — | IC50 | = | 180.0 | nM | 6.75 |
| CHEMBL5430984 | — | IC50 | = | 180.0 | nM | 6.75 |
| CHEMBL4856219 | — | IC50 | = | 200.0 | nM | 6.70 |